Figure 2.

Proliferation and cytokine production in CD8⁺ T cells during P3 infection. C57BL/6 mice (female, 6-8 weeks old) were i.v. injected with 5×10⁴ PFUs of P3, and splenocytes were analyzed every 7 days from 0-21 dpi via flow cytometry. (A) Flow cytometry plots and frequencies of PD-1⁺TIM-3⁺ cells within CD8⁺ T cells in the spleen during P3 infection. (B, C, D, E, F, G, H) Representative flow cytometry histograms (left) and MFI (right) of IL-2, IFN-γ, TNF-α, Ki-67, perforin, granzyme B, and CD107a staining in different CD8⁺ T cell subpopulations are shown. (I) A representative flow cytometry histogram (left) and MFI (right) of IL-21r staining of different CD8⁺ T cells subpopulations are shown. Representative results from repeated experiments are shown. The data are presented as arithmetic means ± SEMs. ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001.