Table 1.
Primary antibodies employed in western blot and blotting strategies.
| Target | Species reactivity | Vendor | Cat. number | Dilution | Transfer Stack |
|---|---|---|---|---|---|
| ACTB | Human | Sigma-Aldrich | A5441 | 1:5 000 | Nitrocellulose |
| ALIX | Human | Santa Cruz Biotechnology | sc-53538 | 1:500 | Nitrocellulose |
| ANXA1 | Human | BD Biosciences | 610066 | 1:5 000 | Nitrocellulose |
| CALR | Human | BD Biosciences | 612136 | 1:250 | Nitrocellulose |
| CD62E | Human | ThermoFischer Scientific | 14062782 | 1:100 | Nitrocellulose |
| CD63 | Human | Millipore | CBL553 | 1:100 | Nitrocellulose |
| CD81 | Human | BD Biosciences | 555675 | 1:250 | Nitrocellulose |
| CD9 | Human | BD Biosciences | 555370 | 1:500 | Nitrocellulose |
| FLOT1 | Human | BD Biosciences | 610820 | 1:500 | Nitrocellulose |
| FLOT2 | Human | BD Biosciences | 610383 | 1:250 | Nitrocellulose |
| FN | Human | BD Biosciences | 610077 | 1:5 000 | PVDF |
| GAPDH | Human | Santa Cruz Biotechnology | sc-47724 | 1:200 | Nitrocellulose |
| GM130 | Human | Santa Cruz Biotechnology | sc-55591 | 1:500 | Nitrocellulose |
| H3 | Human | Cell Signaling Technologies | 4499S | 1:2 000 | Nitrocellulose |
| HSP70 | Human | BD Biosciences | 610607 | 1:250 | Nitrocellulose |
| LAMB2 | Human | BD Biosciences | 610722 | 1:250 | PVDF |
| LMNB1 | Human | Santa Cruz Biotechnology | sc-374015 | 1:250 | PVDF |
| SSEA-4 | Human | BD Biosciences | 560073 | 1:500 | Nitrocellulose |
| TRA1-60 | Human | Abcam | ab16288 | 1:250 | PVDF |
| TRA1-81 | Human | Abcam | ab16289 | 1:250 | PVDF |
| UQCRC1 | Human | Abcam | ab110252 | 1:1 000 | Nitrocellulose |