Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Oct 30;16(12):3033–3056. doi: 10.1038/s44321-024-00157-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

PMC Copyright notice

Figure EV1 — (A) Representative image of ADAM12 immunofluorescence staining (ADAM12 antibody, Invitrogen, PA5-50594) on the healthy pancreas and PDAC tumor tissue from KPPC transgenic mice. (Scale bar 100 μm). (B) Representative images of Sirius Red/Fast Green staining on subcutaneous KP2 PDAC sections from v-CTRL mice and v-A12 mice. (Scale bare 100 μm). (C) Representative image of ADAM12 immunostaining (ADAM12 antibody, Invitrogen, PA5-50594) on PDAC tumor sections from subcutaneous KP2 PDAC grafts. (Scale bar 100 μm; for the zoom-in picture, the scale bar is 50 μm). (D) Representative images (left) and quantitative analysis (right) of ADAM12⁺ cell characterization, ADAM12⁺ CAFs were indicated as ADAM12⁺/α-SMA⁺ (upper, left) and ADAM12⁺/PDGFR-β⁺ (middle, left), and ADAM12⁺ tumor cells were indicated as ADAM12⁺/CK19⁺ (bottom, left) co-expression on subcutaneous KP2 PDAC tumors. (n = 12 fields of view from four mice. Data were presented as mean ± SEM. Scale bar is 200 μm, and the scale bar of the zoomed-in picture is 50 μm). (E) Vector maps of CTRL vaccine (v-CTRL) and ADAM12 vaccine (v-A12). (F) Quantitative analysis of ADAM12⁺/α-SMA⁺ (left, left), ADAM12⁺/PDGFR-β⁺ (left, middle), and ADAM12⁺/CK19⁺ (left, right) co-expression on PDAC tumors from control-vaccinated mice (v-CTRL) and ADAM12-vaccinated mice (v-A12). Data were represented as the number of cells per high power field (HPF). Representative double positive cells were indicated by white arrows respectively. (n = 12–16 in v-CTRL, n = 8–9 in v-A12. Data were presented as mean ± SEM. Statistical test: multiple t-test. Scale bar 50 μm).