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. 2024 Oct 30;16(12):3033–3056. doi: 10.1038/s44321-024-00157-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV5 — (A) Representative immunofluorescence staining images (left) and quantitative analysis (right) of ADAM12⁺/α-SMA⁺ (left, left), ADAM12⁺/PDGFR-β⁺ (left, middle) and ADAM12⁺/CK19⁺ (left, right) co-expression on PDAC tumors from control-vaccinated mice (v-CTRL, n = 5 mice) and ADAM12-vaccinated mice (v-A12, n = 7 mice). Data were represented as the number of cells per high power field (HPF). Representative double positive cells were indicated by white arrows respectively. (n = 5 in v-CTRL, n = 7 in v-A12. Data were presented as mean ± SEM. Statistical test: unpaired two-tailed Student’s t-test. Scale bar 50 μm). (B) Quantitative FACS analysis of the absolute number of T cells, CD8⁺ T cells, CD62L^-CD8⁺ effector T cells, CD4⁺ T cells, and CD62L^-CD4⁺ effector T cells in spleens from control-vaccinated mice (v-CTRL) and ADAM12-vaccinated mice (v-A12). (n = 12 mice in v-CTRL, n = 14 mice in v-A12. *n = 10 mice in v-CTRL in the CD62L^-CD8⁺ effector T cell population because of two outliers. Data were presented as mean ± SEM. Statistical test: unpaired two-tailed Student’s t-test.). (C) Quantitative analysis of the number of neutrophils (left) and macrophages (right) per 1 M single cells in PDAC tumors from control-vaccinated mice (v-CTRL) and ADAM12-vaccinated mice (v-A12). (n = 10 mice in v-CTRL, n = 11 mice in v-A12. Data were presented as mean ± SEM. Statistical test: unpaired two-tailed Student’s t-test.). (D) Representative images of CD8⁺ T cell (left) and CD4⁺ T cell (right) localization, and quantitative analysis of CD8⁺ T cell and CD4⁺ T cell population at the edge of PADC tumor tissue (right, upper) and the interior of PDAC tumor tissue (right, bottom). (n = 5 mice in v-CTRL, n = 5 mice in v-A12. Data were presented as mean ± SEM. Statistical test: unpaired two-tailed Student’s t-test. Scale bar 50 μm). (E) Representative hepatic micrometastasis (area indicated within the white dotted outline) image with H&E staining, scale bar 50 µm.