Reagents and tools table
| Reagent/resource | Reference or source | Identifier or catalog number |
|---|---|---|
| Experimental models | ||
| C57BL/6JRj (M.musculus) | JANVIER LABS | |
| NIH 3T3 cell line | ATCC | ATCC number: CRL-1658 |
| YAC-1 cell line | Veronika Sexl lab provided | N/A |
| MutuDC1940 cell line | Hans Acha-Orbea lab provided | Cat# ACC635 |
| KP2 cell line | David DeNardo lab provided | N/A |
| KPC cell line | Ana Hennino lab provided | N/A |
| pLV.EF1.TurboGFP.WPRE-CTRL vaccine (v-CTRL) | Flash Therapeutics | N/A |
| pLV.EF1.Adam12.T2A.TurboGFP – ADAM12 vaccine (v-A12) | Flash Therapeutics | N/A |
| Recombinant DNA | ||
| pcDNA.EF1.TurboGFP | Flash Therapeutics | N/A |
| pcDNA.EF1.Adam12.T2A.TurboGFP | Flash Therapeutics | NM_007400.2 |
| Antibodies | ||
| Rabbit anti-α-SMA (1:100) | Abcam | Cat# ab5694 |
| Cy3 conjugated mouse anti-α-SMA (1:1000) | Sigma-Aldrich | Cat# C6198 |
| Rabbit anti-ADAM12 (1:100) | Thermo Fisher | Cat# PA5-50594 |
| Goat anti-ADAM12 (1:50) | Novus Biologicals | Cat# NB300-889 |
| Rabbit anti-PDGFR-β (1:100) | Cell Signaling Technology | Cat# 3169 |
| Rat anti-CK19 (10 ng/mL) | DSHB | Cat# TROMA-III |
| Rabbit anti-GLUT1 (1:100) | Abcam | Cat# ab652 |
| Rat anti-CD31 (1:10) | Dianova | Cat# DIA-31 |
| Rabbit anti-CD4 (1:100) | Abcam | Cat# ab183685 |
| Rabbit anti-CD8a (1:100) | Abcam | Cat# ab217344 |
| Sheep anti-Ki67 (1:100) | R&D system | Cat# AF7649 |
| Rabbit anti-Cleaved Caspase 3 (1:200) | Cell Signaling Technology | Cat# 9661 |
| Mouse anti-Pimonidazole (1:50) | HPI | Cat# HP1-XXX |
| Donkey anti-rabbit IgG (H + L) Cy3 (1:300) | Jackson Immunoresearch Labs | Cat# 711-165-152 |
| Donkey anti-goat IgG (H + L) Alexa Fluor 488 (1:500) | Thermo Fisher Scientific | Cat# A11055 |
| Goat anti-rabbit IgG (H + L) Alexa Fluor 488 (1:500) | Thermo Fisher Scientific | Cat# A11034 |
| Donkey anti-sheep IgG Cy3 (1:300) | Jackson Immunoresearch Labs | Cat# 713-165-003 |
| Donkey anti-rat IgG (H + L) Alexa Flour 647 (1:500) | Thermo Fisher Scientific | Cat# A78947 |
| Goat anti-rat IgG (H + L) Alexa Flour 568 (1:500) | Thermo Fisher Scientific | Cat# A11077 |
| Goat anti-mouse antibody Cy3 (1:300) | Jackson Immunoresearch Labs | Cat# 115-165-003 |
| Rat anti-CD8a (53-6.7) (1:100) | Biolegend | Cat# 100730 |
| Rat anti-CD62L (MEL-14) (1:100) | Biolegend | Cat# 104436 |
| Rat anti-CD44 (1M7) (1:100) | Biolegend | Cat# 103049 |
| Rat anti-CD45 (30-F11) (1:100) | Biolegend | Cat# 564225 |
| Rat anti-CD4 (GK1.5) (1:100) | BD Biosciences | Cat# 563790 |
| Rat anti-CD4 (GK1.5) (1:100) | BD Biosciences | Cat# 565974 |
| Hamster anti-CD3 (145-2C11) (1:100) | BD Biosciences | Cat# 562286 |
| Rat anti-F4/80 (BM8) (1:100) | Biolegend | Cat# 123128 |
| Hamster anti-CD11c (N418) (1:100) | Biolegend | Cat# 117310 |
| Rat anti-Ly6G (1A8) (1:100) | BD Biosciences | Cat# 746448 |
| Rat anti-Ly6C (HK1.4) (1:100) | Biolegend | Cat# 128035 |
| Rat anti-CD45 (30-F11) (1:100) | Biolegend | Cat# 103132 |
| Rat anti-CD11b (M1/70) (1:100) | BD Biosciences | Cat# 564443 |
| Rat anti-Siglec-F (E50-2440) (1:100) | BD Biosciences | Cat# 562757 |
| Rat anti-CD45 (30-F11) (1:100) | Biolegend | Cat# 103149 |
| Hamster anti-CD69 (1:100) | BD Biosciences | Cat# 551113 |
| Rat anti-CD8a (53-6.7) (1:100) | Biolegend | Cat# 100738 |
| Rat anti-CD62L (MEL-14) (1:100) | Biolegend | Cat# 104407 |
| Rat anti-CD44 (IM7) (1:100) | Biolegend | Cat# 103026 |
| Rat anti-CD4 (RM4-5) (1:100) | Biolegend | Cat# 100548 |
| Rat anti-CD3 (17A2) (1:100) | Invitrogen | Cat# 50-0032-80 |
| Rat anti-CD45 (30-F11) (30-F11) | Biolegend | Cat# 103151 |
| Hamster anti-CD11c (N418) (1:100) | Biolegend | Cat# 117302 |
| Rat anti-F4/80 (BM8) (1:100) | Biolegend | Cat# 123114 |
| Rat anti-Ly6G (1A8) (1:100) | Biolegend | Cat# 127645 |
| Rat anti-Ly6C (HK1.4) (1:100) | Biolegend | Cat# 128008 |
| Rat anti-CD11b (M1/70) (1:100) | Biolegend | Cat# 101230 |
| Anti-CD3ε (145-2C11) (1:100) | Biolegend | Cat# 100359 |
| Anti-CD28 (37.51) (1:100) | Biolegend | Cat# 102121 |
| Anti-CD31 (MEC13.3) (1:100) | Biolegend | Cat# 102514 |
| Oligonucleotides and other sequence-based reagents | ||
| 16s forward primer: 5′-AGATGATCGAGCCGCGC-3′ | This paper | Fig. EV2E, EV4D |
| Olfm4 forward primer: 5′-GCCACTTTCCAATTTCAC-3′ | This paper | Fig. EV2E, EV4D |
| Krt19 forward primer: 5′- AATGGCGAGCTGGAGGTGAAGA-3′ | This paper | Fig. EV2E, EV4D |
| Chemicals, enzymes, and other reagents | ||
| Mouse Tumor Dissociation Kit | Miltenyi Biotech | Cat# 130-096-730 |
| Murine IFN-γ ELISpot assay | Diaclone | Cat# 862.031.010S |
| CD8a+ (Ly-2) T Cell Isolation Kit (mouse) | Miltenyi Biotech | Cat# 130-104-075 |
| CD4+ (L3T4) T cell isolation Kit (mouse) | Miltenyi Biotech | Cat# 130-117-043 |
| High-Capacity cDNA Reverse Transcription Kit | Applied BiosystemTM | Cat# 4368814 |
| LightCycler® 480 SYBR Green I Master MIX | Roche | Cat# 04887352 |
| TLR9 agonist CpG oligodeoxynucleotides | InvivoGen | Cat# ODN1826 |
| Incomplete Freund’s adjuvant | Sigma-Aldrich | Cat# F5506 |
| Bovine Serum Albumin | Sigma-Aldrich | Cat# A9418 |
| HBSS | Thermo Fisher Scientific | Cat# 14025092 |
| Software | ||
| FlowJo Software (version 10.4.2) | Three Star | https://www.flowjo.com/ |
| ImageJ (version 1.54 f) | NIH | https://imagej.nih.gov/ij/ |
| Ilastik (version 1.4.1-b6) | Ilastik | https://www.ilastik.org/ |
| Graphpad Prism (version 10) | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| AID EliSpot Software | AID | N/A |
| Affinity Designer 2 | Affinity | https://affinity.serif.com/ |
| Other | ||