Table 2.

Parameters resulting from the least-squares fit of the SV samples

Model fit parameter	SV Komorowski et al.	SV Castorph et al.	SV sucrose buffer	SV TRIS buffer	Unit
${\rho }_{\text{in}},{\rho }_{\text{out}}$	46.8	46.8	35.3	46.8	${\mathrm{e}}^{-}{\text{nm}}^{-3}$
${\rho }_{\text{tail}}$	−28.8	−28.8	−40.3	−28.8	${\mathrm{e}}^{-}{\text{nm}}^{-3}$
$t_{\text{in}},t_{\text{out}}$	1.6	1.79	1.86	1.62	nm
$t_{\text{tail}}$	2.33	2	0.96	1.07	nm
$R_g^{\text{in}}$	2.51	2.86	2.3	1.7	nm
$R_g^{\text{out}}$	4.38	5.3	4.8	5.01	nm
$N_c^{\text{in}}/\left(4\pi {\left(R-D-R_g^{\text{in}}\right)}^2\right)$	0.0179	0.0084	0.027	0.04	${\text{nm}}^{-2}$
$N_c^{\text{out}}/\left(4\pi {\left(R+R_g^{\text{in}}\right)}^2\right)$	0.00136	0.0009	0.00097	0.0009	${\text{nm}}^{-2}$
${\rho }_c$	52.1	52.1	40.6	52.1	${\mathrm{e}}^{-}{\text{nm}}^{-3}$
R	16.95	16.95	16.95	16.95	nm
${\sigma }_{\mathrm{R}}$	3.92	3.92	3.92	3.92	nm
Amplitude	248.19	248.19	253.9	237.5	Arb. units
${\mathrm{R}}_{\text{large}}$	277.84	328.58	280.15	297.92	nm
${\sigma }_{\mathrm{R},\text{large}}$	40.8	82.5	51.7	82.33	nm
${\text{Amplitude}}_{\text{large}}$	0.43	1.22	0.84	0.99	Arb. units
Scale	1.0097	0.0838	0.77	0.095	–
Constant background	0.00109	0.00019	0.0028	$-9.66\times {10}^{-6}$	1/mm
${\chi }_{\text{red}}^2$	67.8	4.99	52.19	18.82

Fit results corresponding to the fits in Fig. 3, with the model described in (13,14). In the model, vesicles are described as polydisperse spherical particles with a mean radius R, standard deviation σ, and amplitude of the Gaussian distribution. To account for contamination by larger membranous particles, a second size distribution with radius $R_{\text{large}}$, width ${\sigma }_{\text{large}}$ and ${\text{amplitude}}_{\text{large}}$ is introduced. The lipid bilayer is described by three Gaussians with amplitudes ${\rho }_i$, which describe the excess electron density (compared with water), while $t_i,i\in \left\{\text{in},\text{out},\text{tail}\right\}$ describe the width of the head and tail regions, respectively. The bilayer is assumed to be symmetric, i.e., $t_{\text{in}}=t_{\text{out}}$ and ${\rho }_{\text{in}}={\rho }_{\text{out}}$. The overall thickness of the bilayer is given by $D_b=\sqrt{2\pi }\left(t_{\text{in}}+t_{\text{tail}}+t_{\text{out}}\right)$. Proteins around the lipid bilayer are described by Gaussian chains with radius of gyration $R_g^i$, copy number $N_c^i,i\in \left\{\text{in},\text{out}\right\}$, and the electron density ${\rho }_c$. All electron densities are given as the density difference to the buffer solution. The parameters for the SV fractions from previous studies can be found in (14). Confidence intervals for the model parameters found in this study are given in the supporting material.