Figure 3. MHC class I-restricted peptides derived from flagellin-related Lachnospiraceae gene families show structural homology with human tumor-associated antigens.

(A) Structural simulation of three candidate epitopes (right of pair) predicted from CR-associated flagellin gene families, determined to have sequence and structural homology to a tumor-associated antigen (TAA) (left of pair) and strong affinity to the same HLA allele as that of the TAA (<100 nM). See also Table S6. For immunohistochemistry results, also see Figure S8A.

(B) Experimental binding affinities of the selected flagellin peptides toward A02:01 (left), B07:02 (center), and B08:01 (right), calculated by titrating different concentrations of the test peptide in the presence of 1 μM of protein and fixed concentration (25 nM) of probe peptide HIV-RT (left), MAGE2 (center), and ELR-IAV (right), respectively. See Table 3.