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Confirmation of transgene in C57BL/6J(mtC57BL/6J) and C57BL/6J(mtFVB)
Sequencing products utilizing primers spanning transgene and insertion site for identification of transgene and site-specific insertion into the ROSA26 locus (n = 6 animals) (A). PCR results confirm the presence of the oATP8 construct within the fourth generation of mouse pups born from transgenic C57BL/6J(mtC57BL/6J) or C57BL/6J(mtFVB) parents (B).
