Figure 3.

Exogenous optimized ATP8 is ubiquitously expressed

Denaturing PAGE western blots from processed liver, brain, heart, skeletal muscle, kidney, and spleen of 12-week-old non-transgenic and transgenic C57BL/6J(mtC57BL/6J) (A) and C57BL/6J(mtFVB) (C) mice expressing the oATP8 protein. The C-terminal FLAG epitope was immunodetected with mouse anti-FLAG antibody against the oATP8 protein. GAPDH was used as a loading control. Approximately twenty-five micrograms of protein was loaded per lane (n = 5 biological replicates). Values are expressed as means ± standard error of the mean. The immunoblot bands of oATP8 were quantified by densitometry analysis (using ImageJ), normalized to GAPDH (B and D).