Fig. 5.

Functional activity of through TVP-virus generated anti-CD38 + TIM3 + CLEC12A CAR T-cells. (A) Histograms showing the median fluorescent intensity of single target positive UM9 cells, expressing no target (UM9-CD38KO) or either CD38, TIM3 or CLEC12A only (UM9-CD38, UM9-TIM3, UM9-CLEC12A, respectively). (B) The cytotoxic activity of anti-CD38 + TIM3 + CLEC12A (38/C/T) CAR T-cells against UM9 cells that express none or only one of the putative CAR target antigens. CAR T-cells or untransduced (UT) T-cells were incubated with target cells in different effector to target (E:T) ratios for 24 h. In all experiments n = 3 and duplicates, error bars represent mean ± SD. Statistical analysis in (B) was performed by two-way analysis of variance (ANOVA) and subsequent multiple comparisons. ns = not significant, * indicates p value < 0.05, ** <0.01 and *** <0.001.