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. 2024 Dec 10;169(1):e16274. doi: 10.1111/jnc.16274

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© 2024 The Author(s). Journal of Neurochemistry published by John Wiley & Sons Ltd on behalf of International Society for Neurochemistry.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Specific staining of mCNPase using fluorescently labelled nanobodies. (a) Epifluorescence images of teased sciatic nerves from wild‐type mice stained with Nb5E, Nb8D, and Nb10E displayed in cyan, nuclei stained with DAPI displayed in magenta. (b) Teased sciatic nerves from mice lacking CNPase display no staining (cyan) and nuclei in magenta. Images were acquired with the same acquisition settings and are equally scaled for display and direct comparison. (c) 2‐Colour 2D STED image using Nb8D‐Ab635p (grey) and voltage‐gated sodium channels (Nav1.6; yellow).