Skip to main content
. 2024 Dec 5;8(2):e202402757. doi: 10.26508/lsa.202402757

Figure 6. RuvBL2 co-expression rescues age related motor impairments in Drosophila pan-neuronally expressing dipeptide repeats.

Figure 6.

(A, B, C, D) The vertical distance climbed 5 s after startle-induced negative geotaxis was recorded in Drosophila pan-neuronally (nSyb-Gal4) co-expressing mKate or Reptin with either mCD8-GFP control (A), PA1000 (B), GR1000 (C), or PR1000 (D), at 7 and 14 DPE. The minimum number of flies in any one group was eight (mean ± SEM, flies were from at least three independent crosses per genotype; one-way ANOVA with Tukey post-test: **P < 0.01, ns, non-significant). (E) The activity of Drosophila pan-neuronally (nSyb-Gal4) co-expressing mKate or Reptin with either mCD8-GFP control, PA1000, GR1000, or PR1000, was assessed at 14 DPE over a 24-h period. The total number moves per hour are presented, with the 12-h dark cycle indicated in grey (mean ± SEM, a minimum of at least six flies were used per group). (F) The total number of moves per day per, of each individual animal for each genotype are presented (mean ± SEM, flies were from at least three independent crosses per genotype; one-way ANOVA with Tukey post-test: *P < 0.05, **P < 0.01, ****P < 0.0001). Flies were from at least three independent crosses per genotype. (G) The total time (in hours) each individual animal was classified as sleeping during daylight hours are presented for each genotype (mean ± SEM, flies were from at least three independent crosses per genotype; one-way ANOVA with Šídák’s multiple comparisons test: *P < 0.05, ****P < 0.0001).