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. 2024 Dec 5;8(2):e202402757. doi: 10.26508/lsa.202402757

Figure 8. RuvBL overexpression reduces transcription of C9orf72 sense DNA.

Figure 8.

(A) HeLa cells transfected with empty vector control (ev), FLAG-RuvBL1, or HA-RuvBL2 were co-transfected with empty vector or with 45 uninterrupted sense GGGGCC repeats (45xG4C2). RuvBL overexpression was confirmed via immunoblot with GAPDH indicating equal loading of samples. (B, C, D) The levels of transcription of the sense repeats (B), GAPDH (C), and endogenous C9orf72 (D) was quantified by RT-qPCR using 18S as a housekeeping gene (mean ± SEM, N = 4 independent experiments; one-way ANOVA with Dunnett’s post-test: ns, non-significant, ****P < 0.0001).