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. 2024 Dec 5;8(2):e202402757. doi: 10.26508/lsa.202402757

Figure S10. Knockdown of RuvBL does not affect 45xG4C2 transcript levels.

Figure S10.

HeLa cells treated with control siRNA (siCtrl), RuvBL1-targeting siRNA (siRuvBL1), or RuvBL2-targeting siRNA (siRuvBL2) were transfected with empty vector control or 45 uninterrupted sense GGGGCC repeats (V5-45xG4C2). (A, B) RuvBL1 transcript levels (A) and RuvBL2 transcript levels (B) were determined via RT-qPCR using 18S as a housekeeping gene. Levels of RuvBL1 and RuvBL2 transcripts are shown relative to the siCtrl sample transfected with empty vector control plasmid. (C) V5-45xG4C2 transcript levels were quantified by RT-qPCR using 18S as a housekeeping gene (mean ± SEM, N = 3 independent experiments; one-way ANOVA with Tukey post-test: ns, non-significant, ****P < 0.0001).