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. 2024 Dec 5;8(2):e202402757. doi: 10.26508/lsa.202402757

Figure S4. Detection of RuvBL1 and RuvBL2 with endogenous antibodies.

Figure S4.

(A, B, C, D, E) HeLa cells transfected with empty vector control (Ctrl), FLAG-RuvBL1, or HA-RuvBL2 (A, B, C, D, E) were co-transfected with empty vector (A), AUG-driven synthetic codon-optimised V5-tagged 100 repeat poly(GA) (B), poly(GR) (C), poly(PR) (D), or V5-tagged 45xG4C2 repeats (E). Samples correspond to samples in Fig 1, and were probed with anti-RuvBL1 antibodies (top panels) and anti-RuvBL2 antibodies (bottom panels) to demonstrate the level of overexpression compared with endogenous RuvBL1/2 levels. α-Tubulin was used to demonstrate equal loading.