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. 2024 Dec 5;8(2):e202402757. doi: 10.26508/lsa.202402757

Figure S6. Knockdown of RuvBL does not affect dipeptide repeat protein levels in vitro.

Figure S6.

HeLa cells treated with control siRNA (siCtrl), RuvBL-targeting siRNA (siRuvBL1), or RuvBL2-targeting siRNA (siRuvBL2) were transfected with empty vector control or 45 uninterrupted sense GGGGCC repeats (V5-45xG4C2). (A, B) RuvBL1 levels (A) and RuvBL2 levels (B) were determined via immuoblot with GAPDH used to indicate equal loading. RuvBL1 and RuvBL2 levels were normalised to GAPDH (mean ± SEM; one-way ANOVA with Tukey post-test: ***P ≤ 0.001, ****P ≤ 0.0001; N = 4 independent experiments). (C) Levels of repeat-associated non-AUG translated poly(GP) dipeptide repeats were determined via MSD-ELISA (one-way ANOVA with Tukey post-test: **P ≤ 0.01, ns, non-significant, N = 4 independent experiments).