(A) Schematic diagram indicating the location of qPCR primers (pink arrows) used to determine V5-45xG4C2 transcript levels. (B) HeLa cells transfected with empty vector control (Ctrl), FLAG-RuvBL1, or HA-RuvBL2 were co-transfected with EGFPC2 plasmid and EGFP transcripts quantified by RT-qPCR using GAPDH as housekeeping gene. EGFP transcript levels are shown relative to the EGFP+Ctrl samples (mean ± SEM, N = 3 independent experiments; one-way ANOVA with Tukey post-test: ns, non-significant, ****P < 0.0001). (C) HeLa cells transfected with empty vector control (Ctrl), FLAG-RuvBL1, or HA-RuvBL2 were co-transfected with 45 uninterrupted sense GGGGCC repeats (V5-45xG4C2). V5-45xG4C2 transcript levels were quantified by RT-qPCR using GAPDH as a housekeeping gene (mean ± SEM, N = 3 independent experiments; one-way ANOVA with Tukey post-test: *P < 0.05, ****P < 0.0001). (B, C, D) GAPDH transcript levels in samples from (B, C) were quantified by RT-qPCR using 18S as a housekeeping gene (mean ± SEM, N = 3 independent experiments; one-way ANOVA with Tukey post-test: all ns, non-significant).