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. 2024 Dec 5;8(2):e202402757. doi: 10.26508/lsa.202402757

Figure S9. The effect of RuvBL overexpression on transcription is not CMV promoter dependent.

Figure S9.

(A) Schematic diagram indicating the location of qPCR primers (pink arrows) used to determine V5-45xG4C2 transcript levels. (B) HeLa cells transfected with empty vector control (Ctrl), FLAG-RuvBL1, or HA-RuvBL2 were co-transfected with EGFPC2 plasmid and EGFP transcripts quantified by RT-qPCR using GAPDH as housekeeping gene. EGFP transcript levels are shown relative to the EGFP+Ctrl samples (mean ± SEM, N = 3 independent experiments; one-way ANOVA with Tukey post-test: ns, non-significant, ****P < 0.0001). (C) HeLa cells transfected with empty vector control (Ctrl), FLAG-RuvBL1, or HA-RuvBL2 were co-transfected with 45 uninterrupted sense GGGGCC repeats (V5-45xG4C2). V5-45xG4C2 transcript levels were quantified by RT-qPCR using GAPDH as a housekeeping gene (mean ± SEM, N = 3 independent experiments; one-way ANOVA with Tukey post-test: *P < 0.05, ****P < 0.0001). (B, C, D) GAPDH transcript levels in samples from (B, C) were quantified by RT-qPCR using 18S as a housekeeping gene (mean ± SEM, N = 3 independent experiments; one-way ANOVA with Tukey post-test: all ns, non-significant).