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. 2024 Nov 13;18(1):wrae231. doi: 10.1093/ismejo/wrae231

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© The Author(s) 2024. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Removal of EhV and corresponding abundances of bacteria (a) as measured by flow cytometry in the high purification experiment (#2), showing increasing isotope enrichment of bacteria-sized organic particles over time (b). Individual protist cells were separated analyzed by nanoSIMS (one representative protist shown in panel c), and the 27 analyzed protist cells exhibited a positive correlation between ¹³C and ¹⁵N enrichment from the isotope labeled EhVs (d). Protist isotope enrichment is also plotted on panel b to compare to bacteria-sized particles.