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. 2024 Sep 23;120(15):1967–1984. doi: 10.1093/cvr/cvae160

Figure 8.

Figure 8

Inhibition of neovascular capillaries prevents AV shunt regression. (A) Representative images of Arpc4-WT (top) and Arpc4-iECKO (bottom) retinas at Day 7 stained for vascular network (CD31, grey). Black arrows: AV shunts; A, artery; V, vein. Scale bar: 200 µm. (B) Quantification of AV shunt prevalence at Day 7 in Arpc4-WT (29 AV sections) and Arpc4-iECKO (16 AV sections) retinas. Each dot represents a mouse retina. P-value from Mann–Whitney test. (C) Representative images of Srf-WT (top) and Srf-iECKO (bottom) retinas at Day 9 stained for vascular network (CD31, grey). Black arrows: AV shunts; A, artery; V, vein. Scale bar: 200 µm. (D) Quantification of AV shunt prevalence at Day 9 in Srf-WT (86 AV sections) and Srf-iECKO (63 AV sections) retinas. Each dot represents a mouse retina. P-value from Mann–Whitney test. (E) Quantification of venous (vein and venous capillaries) EC volume at Day 0, Day 1, Day 3, and Day 7 and of non-OIR mouse retinas corresponding to time points Day 0 (P11) and Day 7 (P18). Each dot represents one EC from Day 0 (42 cells, 3 pups); Day 1 (41 cells, 3 pups); Day 3 (27 cells, 3 pups); Day 7 (21 cells, 5 pups); non-OIR Day 0 (37 cells, 5 pups); and non-OIR Day 7 (37 cells, 5 pups). P-values from Kruskal–Wallis test with Dunn’s correction for multiple comparisons. (F) Model describing AV shunt regression in OIR protocol.