Fig. 11.

The accumulation of KNS3 and its two homologs is mutually dependent. (A) Immunoblotting of KNS3, KNSTH1, and KNSTH2 proteins in microsomal fractions. wild-type (WT), kns3-3, knsth1-1, and knsth2-1 plants were grown on solid medium containing 30 μM B for 3 weeks. Approximately 60 plants were used as one sample for microsome preparation. Immunoblot analysis was performed using anti-KNS3, anti-KNSTH1, and anti-KNSTH2 antibodies. (B) Densitometric quantification. Data represent the mean ±SD of three independent experiments.