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. 2024 Oct 30;75(22):7046–7065. doi: 10.1093/jxb/erae380

Fig. 6.

Fig. 6.

mCherry-KNS3 is localized in the endoplasmic reticulum and Golgi. (A) Topology of KNS3 with an N-terminal signal peptide and a transmembrane domain. In the mCherry-KNS3 construct, mCherry was inserted after the signal peptide. (B) mCherry-KNS3 and GFP-HDEL or Man1-GFP expressed in protoplasts from wild-type (Col-0) Arabidopsis leaf mesophyll cells using polyethylene glycol-mediated transformation. (C) mCherry-KNS3 and GFP-HDEL, or ST-YFP in N. benthamiana leaf epidermal cells after agroinfiltration. Scale bars=5 μm.