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. 2024 Oct 21;39(12):2743–2753. doi: 10.1093/humrep/deae240

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© The Author(s) 2024. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Characterization of cell types associated with secondary follicles. (A) Immunofluorescence for DDX4 (green), AMH (yellow), and KRT19 (magenta) in secondary follicles present in cryo-thawed cOVA, cryo-thawed tOVA, and freshly isolated tOVA before (D0) and after culture. Dashed line highlights secondary follicles. Scale bar is 50 µm. (B) Immunofluorescence for ACTA2 (green) and CD68 (magenta) in the stromal tissue surrounding secondary follicles present in cryo-thawed cOVA, cryo-thawed tOVA, and freshly-isolated tOVA before (D0) and after culture. Dashed line highlights secondary follicles. Scale bar is 50 µm. Mc_D8, culture after McLaughlin et al. (2018) for 8 days; Xu_W1/W3, culture after Xu et al. (2021) for 1/3 weeks.