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Plasmid combinations, number of wheat lines containing the Cas9 gene, and number of lines showing alteration in the γ- and ω-gliadin region
| Plasmid combination | Target gliadins | Cas9 PCR+ lines | Lines edited |
|---|---|---|---|
| pSSLGamma16 | γ-Gliadins | 13 | 5 |
| pSSLGamma17 | γ-Gliadins | 8 | 0 |
| pSSLOmega8 | ω-Gliadins; γ-gliadins | 24 | 8 |
| pSSLOmega9 | ω-Gliadins; γ-gliadins | 3 | 3 |
| pSSLOmega9+pSSLGamma17 | γ-Gliadins, ω-gliadins | 11 | 4 |
