Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) | B-95.ΔA E. coli | Addgene | Bacterial strain #197933 | |
| Recombinant DNA reagent | pDule2-Mj Acd A9 (plasmid) | Addgene | Plasmid #197652 | |
| Recombinant DNA reagent | MBP-S361TAG -ctermSthK-TEV-TWS.pETM11 (plasmid) | Addgene | Plasmid #231098 | |
| Recombinant DNA reagent | MBP- S361TAG -V416C-ctermSthK-TEV-TWS.pETM11 (plasmid) | Addgene | Plasmid #231099 | |
| Recombinant DNA reagent | MBP-Q364TAG -ctermSthK-TEV-TWS.pETM11 (plasmid) | Addgene | Plasmid #231100 | |
| Recombinant DNA reagent | MBP- Q364TAG -R417C-ctermSthK-TEV-TWS.pETM11 (plasmid) | Addgene | Plasmid #231101 | |
| Software, algorithm | FDlifetime_17_Igor_procedures.ipf | Zagotta, 2021 | https://github.com/zagotta/FDlifetime_program |