Table 1. Calculated energetics of the four state model.
Ligand condition | Ionic strength (mM KCl) | ∆G (kcal/mol) | ∆∆G (kcal/mol) |
---|---|---|---|
Apo | 150 | 1.14±0.03 | -- |
500 | 1.2±0.03 | -- | |
cAMP | 150 | -- | -- |
500 | <–2.7±0 | <–3.9±0.03 | |
cGMP | 150 | 0.67±0.03 | –0.47±0.04 |
500 | 0.38±0.02 | –0.82±0.04 |
Energies are reported in mean values ± SEM.