Table 1. Aurora spectral panel and antibodies list.
| Antigen | Clone | Fluorochrome | Company, catalogue number | Dilution |
|---|---|---|---|---|
| Live/dead | – | Blue | Thermo Fisher, L23105 | 1:1000 |
| CD45 | 30-F11 | AF532 | Thermo Fisher, 58-0451-80 | 1:50 |
| CD19 | 1D3 | SB436 | Thermo Fisher, 62-0193-80 | 1:50 |
| CD3 | 17A2 | eF450 | BioLegend, 48-0032-80 | 1:50 |
| NK1.1 | PK136 | BV650 | BioLegend, 108735 | 1:25 |
| CD8a | 53–6.7 | BV570 | BioLegend, 100739 | 1:25 |
| CD4 | RM4-5 | BV510 | BD Biosciences, 100553 | 1:100 |
| CD25 | 7D4 | AF647 | BD Biosciences, 563598 | 1:100 |
| FOXP3 | FJK-16s | PE | Thermo Fisher, 12-5773-80 | 1:20 |
| CD206 | C068C2 | BV785 | BD Biosciences, 141729 | 1:25 |
| F4/80 | T45-2342 | BV480 | BD Biosciences, 565635 | 1:100 |
| CD80 | REA983 | APC | Miltenyi Biotech, 130-116-46 | 1:100 |
| CD279 (PD-1) | J43 | PE-Cy7 | Thermo Fisher, 25-9985-82 | 1:25 |
| CD195 (CCR5) | REA354 | VB-FITC | Miltenyi Biotech, 30-105-141 | 1:20 |
| CD11b | M1/70 | AF700 | BioLegend, 101222 | 1:100 |
| Ly6C | HK1.4 | PE-Dazzle | BioLegend, 128043 | 1:200 |
| Ly6G | 1A8 | BV421 | BioLegend, 127627 | 1:25 |