Figure 2.

Associations between the presence of known non-KRAS driver mutations, TMB, and PD-L1 in NSCLC tumors. Differences in tumor mutational burden (TMB; in mutations/Mb) and PD-L1 tumor proportion score (TPS) were tested between NSCLC tumors with known driver/non-driver mutations within (A) ALK, (B) EGFR, (C) MET, (D) BRAF, (E) ROS1, and (F) ERBB2. For plots of TMB (top), each dot in the box-violin plots represents an individual tumor from a unique patient falling into a particular variant group (X-axis) and is plotted along the Y-axis based on its log-transformed TMB value (non-transformed TMB value of each transformed value shown in parentheses on the Y-axis for clarity). The bottom, middle, and top horizontal boundaries of each box in the box-violin plots represent the first, second (median), and third quartiles of the data. The lines extending from the two ends of each box represent 1.5x outside the interquartile. Points beyond the lines are considered outliers. The width of the grey-shaded regions around the boxes represents the density of the data points, where wider areas correspond to higher data point density. Values listed under the box-violin plots are the median for the group. For plots of PD-L1 TPS level (bottom), each section of the bar plots represents the percent of tumors falling in a particular variant group (X-axis) that were deemed to be negative (<1%), low (1 - 49%), or high (≥50%) for PD-L1 TPS. Differences in TMB and PD-L1 between groups were tested using linear regression and penalized likelihood ratio tests, respectively, adjusting for NSCLC histology. Results of testing can be found in Supplementary Table S4 . *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001.