Figure 1.

Generation of CD19 CAR-UESC and induction of CD19 CAR-UiNK. (A) Schematic diagram of CD19 CAR-UiNK generation and functional assay. (B) Targeting strategy for the knockout of B2M gene in human ESCs. The exons of the B2M gene are shown as blue boxes. Two gRNA sequences, gRNA1 and gRNA2, are marked in red. (C) Schematic representation of the transposon vector encoding the CD19 CAR. (D) Schematic representation of the transposon vector encoding the HLA-E single chain trimer. (E) Representative flow cytometry plots showing the expression of HLA-ABC, HLA-E, and CD19 CAR in ESC and CD19 CAR-UESC. (F) Schematic diagram of CD19 CAR-UiNK cell induction. (G) Representative flow cytometry plots of CD19 CAR-UiNK cells (CD45⁺CD3^–CD56⁺CD16^+/–HLA-ABC^–HLA-E⁺CD19 CAR⁺) on Day 27.