Table 2.
Map detection and quantification in feces and tissues in challenged animals.
| Group | Animal | Fecal shedding (fg Map-DNA/g feces) | Tissue bacterial load (fg Map-DNA/g tissue) | IHC and ZN* | Culture | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 mpi | 5 mpi | 10 mpi | Group mean (by 10 mpi) | DJPP | JLN | DI | Mean | Group mean | DJPP | JLN | DI | DJPP | JLN | DI | ||
| OV-INF | 21 | 0.00 | 0.00 | 1.15 | 1632.9 ± 1617.78 | 1.56 | 0.00 | 0.33 | 0.63 | 1362.1 ± 1270.46 | − | − | − | − | − | − |
| 22 | 0.00 | 39.01 | 29.95 | 325.11 | 224.14 | 0.00 | 183.08 | + | − | − | − | + | + | |||
| 23 | 0.00 | 731.79 | 4867.71 | 1964.79 | 438.28 | 9304.25 | 3902.44 | + | − | +* | + | + | + | |||
| IDV-INF | 41 | 0.22 | 0.00 | 66.33 | 757.85 ± 722.16 | 771.38 | 9.56 | 0.00 | 260.31 | 131.89 ± 101.18 | + | − | + | − | + | + |
| 42 | 0.04 | 813.95 | 2200.43 | 392.30 | 3.98 | 8.85 | 135.05 | + | − | + | + | − | + | |||
| 43 | 0.00 | 0.00 | 6.80 | 0.00 | 0.90 | 0.00 | 0.30 | − | − | − | − | − | − | |||
| SCV-INF | 61 | 0.00 | 0.00 | 1.46 | 17.78 ± 17.06 | 0.50 | 0.05 | 1.57 | 0.70 | 26.31 ± 28.36 | − | − | + | − | − | − |
| 62 | 0.00 | 1.97 | 0.00 | 0.62 | 8.01 | 1.61 | 3.41 | − | − | − | − | − | − | |||
| 63 | 0.00 | 2.44 | 51.87 | 105.58 | 17.86 | 101.07 | 74.83 | + | − | − | + | − | + | |||
| NV-INF | 81 | 0.64 | 0.00 | 5.79 | 56.42 ± 14.75 | 2.86 | 0.00 | 0.65 | 1.17 | 69.27 ± 127.12 | − | − | − | − | − | − |
| 82 | 0.00 | 0.94 | 83.41 | 246.72 | 9.66 | 0.00 | 85.46 | +* | − | − | − | − | + | |||
| 83 | 0.27 | 0.00 | 80.06 | 326.17 | 14.01 | 23.33 | 121.17 | + | + | + | + | − | + | |||
| 84 | 1.67 | 17.90 | 11.29 | 23.20 | 1.57 | 2.72 | 9.16 | − | − | + | − | − | + | |||
| 85 | 0.06 | 5.33 | 84.66 | 889.58 | 6.41 | 3.96 | 299.99 | +* | − | + | − | − | − | |||
| 86 | 0.00 | 0.00 | 0.00 | 0.00 | 1.56 | 0.00 | 0.52 | − | − | − | − | − | − | |||
| 87 | 0.00 | 134.14 | 7.60 | 2793.89 | 19.86 | 0.00 | 937.92 | + | − | − | − | − | − | |||
Quantification of Map fecal shedding by 1, 5, and 10 mpi (months post-infection) and tissue bacterial load at the end of the experiment through qPCR, expressed as fg Map-DNA/g tissue. Map detection in tissues through the ZN (Ziehl–Neelsen) staining, IHC, and bacteriological culture. *Positive samples to ZN staining. DJPP (distal jejunal Peyer’s patch), JLN (jejunal lymph node), and DI (distal ileum).