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. 2024 Nov 27;15:1465185. doi: 10.3389/fimmu.2024.1465185

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Copyright © 2024 Nakabo, Sandoval-Heglund, Hanata, Brooks, Hoffmann, Zhang, Ambler, Manna, Poncio, Hasni, Islam, Dell’Orso and Kaplan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemistry staining for the expression of April in mouse bone marrow. (A) Representative lower magnification (0.82×) images of IMQ-treated WT and Bmal1^Mye−/− sternum bone marrow. April was stained with anti-mouse April antibody. (B) Higher-magnification (100×) images. April expressed in immature neutrophils (arrowhead) is increased in IMQ-treated Bmal1^Mye−/− . (C) April levels in whole bone marrow were quantified with ImageJ and compared. WT; n=5, Bmal1^Mye−/− ; n=5, WT + IMQ; n=9, Bmal1^Mye−/− + IMQ; n=11. The statistical analysis was done using Mann–Whitney test. *p<0.05, ns, not significant.