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. 2024 Nov 27;15:1491808. doi: 10.3389/fimmu.2024.1491808

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Copyright © 2024 Zhu, Xiao, Hou, Huang, Xu, Guo, Dong, Xu, Zhang and Gu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Functional experiments were performed to explore the biological significance of PLOD1. (A) The protein levels of PLOD1 were analyzed by the western blot in 5637, T24, U3, J82, and SV-HUC1 cells. (B) PLOD1 knockdown efficiency was evaluated by the western blot. (C, D) The CCK-8 and colony formation assays demonstrated that down-regulation of PLOD1 inhibited cell proliferation. (E) Wound-healing assays detected the effects of knockdown of PLOD1 on cell migration. (F) The effect of knockdown of PLOD1 on BLCA cell migration based on transwell assays. *P < 0.05, **P < 0.01, ***P < 0.001.