Table 1.
Primer sequences used in this study.
| Target gene | Primer | Oligonucleotide sequences (5′- 3′) | Amplicon size (bp) | References |
|---|---|---|---|---|
| IS1111 | QBT1 | TATGTATCCACCGTAGCCAGTC | 687 | (31) |
| QBT2 | CCCAACAACACCTCCTTATTC | |||
| QBTN3 | AAGCGTGTGGAGGAGCGAACC | 440 | (32) | |
| QBTN4 | CTCGTAATCACCAATCGCTTCGTC | |||
| 16S rRNA | 16S-F1 | CGTAGGAATCTACCTTRTAGWGG | 624-627 | (33) |
| 16S-F | TGAGAACTAGCTGTTGGRRAGT | |||
| 16S-R | GCCTACCCGCTTCTGGTACAATT | |||
| COX2 | COX2F | CAACCCTGAATACCCAAGGA | 397 | (34) |
| COX2R | GAAGCTTCTGATAGGCGGGA | |||
| COX5 | COX5F | CAGGAGCAAGCTTGAATGCG | 395 | |
| COX5R | TGGTATGACAACCCGTCATG | |||
| COX18 | COX18F | CGCAGACGAATTAGCCAATC | 557 | |
| COX18R | TTCGATGATCCGATGGCCTT | |||
| COX20 | COX20F | GATATTTATCAGCGTCAAAGCAA | 631 | |
| COX20R | TCTATTATTGCAATGCAAGTGG | |||
| COX22 | COX22F | GGGAATAAGAGAGTTAGCTCA | 383 | |
| COX22R | CGCAAATTTCGGCACAGACC | |||
| COX37 | COX37F | GGCTTGTCTGGTGTAACTGT | 463 | |
| COX37R | ATTCCGGGACCTTCGTTAAC | |||
| COX51 | COX51F | TAACGCCCGAGAGCTCAGAA | 674 | |
| COX51R | GCGAGAACCGAATTGCTATC | |||
| COX56 | COX56F | CCAAGCTCTCTGTGCCCAAT | 479 | |
| COX56R | ATGCGCCAGAAACGCATAGG | |||
| COX57 | COX57F | TGGAAATGGAAGGCGGATTC | 617 | |
| COX57R | GGTGGAAGGCGTAAGCCTTT | |||
| COX61 | COX61F | GAAGATAGAGCGGCAAGGAT | 611 | |
| COX61R | GGGATTTCAACTTCCGATAGA |