Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1981 Aug 15;198(2):379–383. doi: 10.1042/bj1980379

Alpha 1-Adrenergic stimulation of Ca2+ mobilization without phosphorylase activation in hepatocytes from phosphorylase b kinase-deficient gsd/gsd rats.

P F Blackmore, J H Exton
PMCID: PMC1163259  PMID: 6948557

Abstract

Phenylephrine, vasopressin and the bivalent cation ionophore A23187 mobilized Ca2+ normally, but failed to activate phosphorylase, in hepatocytes from gsd/gsd rats with a deficiency of liver phosphorylase b kinase. These data provide strong evidence that phosphorylase b kinase is the site of action of the Ca2+ mobilized intracellularly during alpha 1-adrenergic activation of phosphorylase in liver cells.

Full text

PDF
381

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aggerbeck M., Guellaen G., Hanoune J. Adrenergic receptor of the alpha 1-subtype mediates the activation of the glycogen phosphorylase in normal rat liver. Biochem Pharmacol. 1980 Feb 15;29(4):643–645. doi: 10.1016/0006-2952(80)90389-5. [DOI] [PubMed] [Google Scholar]
  2. Althaus-Salzmann M., Carafoli E., Jakob A. Ca2+, K+ redistributions and alpha-adrenergic activation of glycogenolysis in perfused rat livers. Eur J Biochem. 1980 May;106(1):241–248. doi: 10.1111/j.1432-1033.1980.tb06015.x. [DOI] [PubMed] [Google Scholar]
  3. Assimacopoulos-Jeannet F. D., Blackmore P. F., Exton J. H. Studies on alpha-adrenergic activation of hepatic glucose output. Studies on role of calcium in alpha-adrenergic activation of phosphorylase. J Biol Chem. 1977 Apr 25;252(8):2662–2669. [PubMed] [Google Scholar]
  4. Babcock D. F., Chen J. L., Yip B. P., Lardy H. A. Evidence for mitochondrial localization of the hormone-responsive pool of Ca2+ in isolated hepatocytes. J Biol Chem. 1979 Sep 10;254(17):8117–8120. [PubMed] [Google Scholar]
  5. Blackmore P. F., Brumley F. T., Marks J. L., Exton J. H. Studies on alpha-adrenergic activation of hepatic glucose output. Relationship between alpha-adrenergic stimulation of calcium efflux and activation of phosphorylase in isolated rat liver parenchymal cells. J Biol Chem. 1978 Jul 25;253(14):4851–4858. [PubMed] [Google Scholar]
  6. Dehaye J. P., Blackmore P. F., Venter J. C., Exton J. H. Studies on the alpha-adrenergic activation of hepatic glucose output. alpha-Adrenergic activation of phosphorylase by immobilized epinephrine. J Biol Chem. 1980 May 10;255(9):3905–3910. [PubMed] [Google Scholar]
  7. El-Refai M. F., Blackmore P. F., Exton J. H. Evidence for two alpha-adrenergic binding sites in liver plasma membranes. Studies with [3H]epinephrine and [3H]dihydroergocryptine. J Biol Chem. 1979 Jun 10;254(11):4375–4386. [PubMed] [Google Scholar]
  8. El-Refai M. F., Exton J. H. Effects of trypsin on binding of [3H]epinephrine and [3H]-dihydroergocryptine to rat liver plasma membranes. Evidence for interconversion of binding sites. J Biol Chem. 1980 Jun 25;255(12):5853–5858. [PubMed] [Google Scholar]
  9. El-Refai M. F., Exton J. H. Subclassification of two types of alpha-adrenergic binding sites in rat liver. Eur J Pharmacol. 1980 Mar 21;62(2-3):201–204. doi: 10.1016/0014-2999(80)90276-9. [DOI] [PubMed] [Google Scholar]
  10. Garrison J. C., Borland M. K., Florio V. A., Twible D. A. The role of calcium ion as a mediator of the effects of angiotensin II, catecholamines, and vasopressin on the phosphorylation and activity of enzymes in isolated hepatocytes. J Biol Chem. 1979 Aug 10;254(15):7147–7156. [PubMed] [Google Scholar]
  11. Garrison J. C. The effects of glucagon, catecholamines, and the calcium ionophore A23187 on the phosphorylation of rat hepatocyte cytosolic proteins. J Biol Chem. 1978 Oct 10;253(19):7091–7100. [PubMed] [Google Scholar]
  12. Hoffman B. B., Dukes D. F., Lefkowitz R. J. Alpha-adrenergic receptors in liver membranes: delineation with subtype selective radioligands. Life Sci. 1981 Jan 19;28(3):265–272. doi: 10.1016/0024-3205(81)90732-3. [DOI] [PubMed] [Google Scholar]
  13. Hoffman B. B., Michel T., Kilpatrick D. M., Lefkowitz R. J., Tolbert M. E., Gilman H., Fain J. N. Agonist versus antagonist binding to alpha-adrenergic receptors. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4569–4573. doi: 10.1073/pnas.77.8.4569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hoffman B. B., Mullikin-Kilpatrick D., Lefkowitz R. J. Heterogeneity of radioligand binding to alpha-adrenergic receptors. Analysis of guanine nucleotide regulation of agonist binding in relation to receptor subtypes. J Biol Chem. 1980 May 25;255(10):4645–4652. [PubMed] [Google Scholar]
  15. Keppens S., Vandenheede J. R., De Wulf H. On the role of calcium as second messenger in liver for the hormonally induced activation of glycogen phosphorylase. Biochim Biophys Acta. 1977 Feb 28;496(2):448–457. doi: 10.1016/0304-4165(77)90327-0. [DOI] [PubMed] [Google Scholar]
  16. Kishimoto A., Mori T., Takai Y., Nishizuka Y. Comparison of calcium-activated, cyclic nucleotide-independent protein kinase and adenosine 3':5'-monophosphate-dependent protein kinase as regards the ability to stimulate glycogen breakdown in vitro. J Biochem. 1978 Jul;84(1):47–53. doi: 10.1093/oxfordjournals.jbchem.a132118. [DOI] [PubMed] [Google Scholar]
  17. Kishimoto A., Takai Y., Mori T., Kikkawa U., Nishizuka Y. Activation of calcium and phospholipid-dependent protein kinase by diacylglycerol, its possible relation to phosphatidylinositol turnover. J Biol Chem. 1980 Mar 25;255(6):2273–2276. [PubMed] [Google Scholar]
  18. Malthus R., Clark D. G., Watts C., Sneyd J. G. Glycogen-storage disease in rats, a genetically determined deficiency of liver phosphorylase kinase. Biochem J. 1980 Apr 15;188(1):99–106. doi: 10.1042/bj1880099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Murphy E., Coll K., Rich T. L., Williamson J. R. Hormonal effects on calcium homeostasis in isolated hepatocytes. J Biol Chem. 1980 Jul 25;255(14):6600–6608. [PubMed] [Google Scholar]
  20. Poggioli J., Berthon B., Claret M. Calcium movements in in situ mitochondria following activation of alpha-adrenergic receptors in rat liver cells. FEBS Lett. 1980 Jun 30;115(2):243–246. doi: 10.1016/0014-5793(80)81178-1. [DOI] [PubMed] [Google Scholar]
  21. Uhing R. J., Janski A. M., Graves D. J. The effect of solvents on nucleotide regulation of glycogen phosphorylase. J Biol Chem. 1979 May 10;254(9):3166–3169. [PubMed] [Google Scholar]
  22. Watts C., Malthus R. S. Liver glycogen synthase in rats with a glycogen-storage disorder. The role of glycogen in the regulation of glycogen synthase. Eur J Biochem. 1980;108(1):73–77. doi: 10.1111/j.1432-1033.1980.tb04697.x. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES