FIGURE 3.

Hotspot predictions in C2_iso1,WT·WRC using the mCSM server (Pires et al. 2013). The five components of the complex are shown as in Figure 2. The hotspots at the protein interfaces are shown as spheres of different colors. Two of them (R87 and D724) are also residues undergoing mutations in ASD (Table 1). Similar results are obtained using the BeAtMuSiC (Dehouck et al. 2013) and the FoldX (Schymkowitz et al. 2005) servers (Figures S6 and S7) and for the C2_iso2–3,WT·WRC complexes (Figure S5).