Figure 5.

Metformin reduced inflammation response via high-mobility group box 1(HMGB1)/toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-κB) pathway in the lung tissue. (A) Representative images of western blot of HMGB1, TLR4, myeloid differentiation primary response protein 88 (MyD88), NF-κB, and interleukin-1 beta in the lung tissues and their quantified calculation of protein expression. (B) mRNA expression levels of HMGB1, TLR4, and NF-κB in lung tissues. The results are presented as mean ± SD. ^aP < 0.05 vs Control group, ^bP < 0.01 vs Control group, ^cP < 0.05 vs IR group, ^dP < 0.01 vs IR group.