FIGURE 1.

The enzymatic role and sequence comparisons between homologues for GAPN. (a) Schematic of the 10 steps of glycolysis that specifically focuses on GAPDH and PGK. GAPN facilitates the direct oxidation of G3P to 3‐PG using NADP⁺ as a cofactor, bypassing the traditional two‐step process. Additionally, the first step of the PPP shunt of glucose‐6‐phosphate‐dehydrogenase (G6PDH) is shown. (b) Sequence homology of GAPN homologues from T. tenax and the three Gram‐positive bacteria, S. pneumoniae, S. pyogenes, and S. mutants. Completely conserved residues are highlighted (blue) and the eight‐residue extension in T. tenax (green). Additional elements of conservation are delineated below these sequences as completely conserved residues (*), along with mostly conserved residues (:), and partially conserved residues (.)