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. 2024 Oct 31;15(12):e02275-24. doi: 10.1128/mbio.02275-24

Fig 4.

Growth curves and spot assays depict OD600 growth measurements for wild-type, ΔsydR, and complemented strains. Lower section depicts colony survival after treatment with hydrogen peroxide and tert-butyl hydroperoxide.

SydR inactivation has no effect on growth or oxidant sensitivity. (A) Growth of WT, ΔsydR, and ΔsydR-ptrp:sydR strains in M9 + casamino acids (M9-CSA) in the presence of various concentrations of NaOCl. (B) Bacterial survival after challenge with H2O2 or tBOOH. WT and ΔsydR strains were grown in M9-CSA to OD600 of 0.1, then incubated in the presence of either H2O2 or tBOOH for 1 h before being serially diluted and spotted on agar plates. Data are representatives of at least three independent experiments.