TABLE 2.
Correlation between BCID GN kit with final culture and phenotypic susceptibility testing results for β-lactams for blood cultures with monomicrobial Gram-negative organisms
| Organism identification by routine methods | BCID identification and number of isolates | Detected AMR genes and number of isolates | Number of isolates with discordant phenotype | ||
|---|---|---|---|---|---|
| Escherichia coli | Escherichia coli, pan-Enterobacterales | 57 | tem | 17 | 1 |
| Pan-Enterobacterales only | 1 | shv | 1 | ||
| ctx-m | 8 | ||||
| ctx-m, tem | 6 | ||||
| ampC | 1 | ||||
| ampC, tem | 1 | ||||
| Nil detected | 24 | 1c | |||
| Klebsiella pneumoniae | Klebsiella spp., pan-Enterobacterales | 31 | shv | 21 | |
| shv, tem | 5 | ||||
| ctx-m, tem | 1 | ||||
| ampC, shv | 1 | ||||
| ctx-m, shv, tem | 2 | ||||
| ampC, shv, tem | 1 | ||||
| Klebsiella variicola | Klebsiella spp., pan-Enterobacterales | 1 | Nil detected | 1 | |
| Enterobacter cloacae complex | Enterobacter spp., pan-Enterobacterales | 1 | Nil detected | 1 | |
| Proteus mirabilis | Proteus spp., pan-Enterobacterales | 3 | Nil detected | 3 | |
| Salmonella Typhi | Salmonella spp., pan-Enterobacterales | 1 | tem | 1 | |
| Serratia spp. | Serratia spp., pan-Enterobacterales | 5 | Nil detected | 5 | |
| Morganella morganii a | Pan-Enterobacterales only | 1 | Nil detected | 1 | |
| Citrobacter koseri a | Pan-Enterobacterales only | 2 | Nil detected | 2 | |
| Citrobacter freundii complexa | Pan-Enterobacterales only | 1 | ampC | 1 | |
| Acinetobacter baumannii complex | Acinetobacter baumannii complex | 4 | Nil detected | 3 | |
| oxa-23 | 1 | ||||
| Pseudomonas aeruginosa | Pseudomonas aeruginosa | 9 | Nil detected | 9 | 1d |
| Stenotrophomonas maltophilia | Stenotrophomonas maltophilia | 3 | Nil detected | –e | N/Af |
| Bacteroides thetaiotaomicronb | No pathogen detected | 1 | N/A | N/A | |
| Elizabethkingia anophelisb | No pathogen detected | 1 | N/A | N/A | |
| Fusobacterium spp.b | No pathogen detected | 1 | N/A | N/A | |
| Parabacteroides distasonisb | No pathogen detected | 1 | N/A | N/A | |
| Pseudomonas otitidisb | No pathogen detected | 1 | N/A | N/A | |
| Roseomonas mucosab | No pathogen detected | 1 | N/A | N/A | |
| Burkholderia cepacia complexb | No pathogen detected | 1 | N/A | N/A | |
| Gabonibacter massiliensisb | No pathogen detected | 1 | N/A | N/A | |
| Porphyromonas speciesb | No pathogen detected | 1 | N/A | N/A | |
a
No species targets available.
b
Not targeted in BCID GN kit.
c
One E. coli isolate with ampicillin and amoxicillin-clavulanic acid resistance.
d
One P. aeruginosa isolate was resistant to all β-lactams.
e
–
, not tested due to intrinsic resistance.
f
N/A, not applicable.