. 2024 Nov 6;62(12):e01169-24. doi: 10.1128/jcm.01169-24

TABLE 4.

Summary of sensitivity and specificity of AMR genes for prediction of phenotypic resistance^c

Organism	Aminopenicillin	shv and/or tem (in isolates without ctx-m/ampC)						Third-generation cephalosporins	ctx-m and/or ampC
Organism	Aminopenicillin	Det	ND	Sens.	Spec.	PPV	NPV	Third-generation cephalosporins	Det.	ND	Sens.	Spec.	PPV	NPV
Escherichia coli (n = 58)	Resistant	18	1	94.7%	95.8%	94.7%	95.8%	Resistant	16	0	100.0%	100.0%	100.0%	100.0%
Escherichia coli (n = 58)	Susceptible	1	23	94.7%	95.8%	94.7%	95.8%	Susceptible	0	42	100.0%	100.0%	100.0%	100.0%
Klebsiella pneumoniae group^a (n = 32)	Resistant	N/A – intrinsic resistance to aminopenicillins						Resistant	5	0	100.0%	100.0%	100.0%	100.0%
Klebsiella pneumoniae group^a (n = 32)	Susceptible	N/A – intrinsic resistance to aminopenicillins						Susceptible	0	26	100.0%	100.0%	100.0%	100.0%
Organism	Gentamicin	aac(6’)-lb						Amikacin	aac(6’)-lb
Organism	Gentamicin	Det	ND	Sens.	Spec.	PPV	NPV	Amikacin	Det.	ND	Sens.	Spec.	PPV	NPV
Escherichia coli (n = 58)	Resistant	2	5	28.6%	98.0%	66.7%	90.9%	Resistant	1	0	100.0%	96.5%	33.3%	100.0%
Escherichia coli (n = 58)	Susceptible	1	50	28.6%	98.0%	66.7%	90.9%	Susceptible	2	55	100.0%	96.5%	33.3%	100.0%
Klebsiella pneumoniae group^a (n = 32)	Resistant	3	1	75.0%	100.0%	100.0%	96.6%	Resistant	0	0	-	90.6%	0.0%	100.0%
Klebsiella pneumoniae group^a (n = 32)	Susceptible	0	28	75.0%	100.0%	100.0%	96.6%	Susceptible	3	29	-	90.6%	0.0%	100.0%
Organism	Penicillin	blaZ (in isolates without mecA)						Cloxacillin	mecA
Organism	Penicillin	Det	ND	Sens.	Spec.	PPV	NPV	Cloxacillin	Det.	ND	Sens.	Spec.	PPV	NPV
Staphylococcus aureus (n = 11)	Resistant	3	0	100.0%	100.0%	100.0%	100.0%	Resistant	7	0	100.0%	100.0%	100.0%	100.0%
Staphylococcus aureus (n = 11)	Susceptible	0	1	100.0%	100.0%	100.0%	100.0%	Susceptible	0	4	100.0%	100.0%	100.0%	100.0%
Coagulase-negative Staphylococcus^b (n = 11)	Resistant	N/A – No break points for penicillin						Resistant	7	0	100.0%	100.0%	100.0%	100.0%
Coagulase-negative Staphylococcus^b (n = 11)	Susceptible	N/A – No break points for penicillin						Susceptible	0	8	100.0%	100.0%	100.0%	100.0%
Organism	Gentamicin	aac(6’)/aph(2”)						Minocycline Sens.	tetM
Organism	Gentamicin	Det	ND	Sens.	Spec.	PPV	NPV	Minocycline Sens.	Det	ND	Sens.	Spec.	PPV	NPV
All Staphylococcus spp.^b (n = 22)	Resistant	8	0	100.0%	100.0%	100.0%	100.0%	Resistant	0	0	-	100.0%	-	100.0%
All Staphylococcus spp.^b (n = 22)	Susceptible	0	15	100.0%	100.0%	100.0%	100.0%	Susceptible	0	22	-	100.0%	-	100.0%
Organism	Erythromycin	ermC						Clindamycin	ermC
Organism	Erythromycin	Det	ND	Sens.	Spec.	PPV	NPV	Clindamycin	Det.	ND	Sens.	Spec.	PPV	NPV
All Staphylococcus spp.^b (n = 22)	Resistant	6	1	85.7%	87.5%	75.0%	93.3%	Resistant	6	0	100.0%	100.0%	100.0%	100.0%
All Staphylococcus spp.^b (n = 22)	Susceptible	2	14	85.7%	87.5%	75.0%	93.3%	Susceptible	0	17	100.0%	100.0%	100.0%	100.0%

^{^a}

Includes 31 K. pneumoniae and one K. variicola isolate.

^{^b}

Detailed breakdown is available in the supplemental material.

^{^c}

Det, detected; ND, not detected: sens., sensitivity; spec., specificity; PPV, positive predictive value; NPV, negative predictive value; N/A, not applicable.