. 2024 Nov 6;62(12):e01169-24. doi: 10.1128/jcm.01169-24

TABLE 5.

Comparison of BCID results with routine testing for polymicrobial cultures

	BCID GP results		BCID GN results		Organism identification by routine methods	Resistant antibiotics
	Identification results	AMR genes detected	Identification results	AMR genes detected	Organism identification by routine methods	Resistant antibiotics
Polymicrobial cultures with Gram-positive organisms only
1	Staphylococcus capitis/hominis, Staphylococcus epidermidis	aac(6’)/aph(2”), blaZ, ermC, mecA, tetK	N/A^a	N/A	Staphylococcus epidermidis	Penicillin, cloxacillin, erythromycin, clindamycin
1		aac(6’)/aph(2”), blaZ, ermC, mecA, tetK	N/A^a	N/A	Staphylococcus capitis	Penicillin, cloxacillin
2	No pathogen detected	Nil detected	N/A	N/A	Staphylococcus haemolyticus
2	No pathogen detected	Nil detected	N/A	N/A	Corynebacterium striatum^b
3	Staphylococcus aureus	mecA, ermC	N/A	N/A	Staphylococcus aureus	Penicillin, cloxacillin, erythromycin, clindamycin
3	Staphylococcus aureus	mecA, ermC	N/A	N/A	Staphylococcus capitis^b	Not performed
4	No pathogen detected	Nil detected	N/A	N/A	Staphylococcus haemolyticus	N/A
4	No pathogen detected	Nil detected	N/A	N/A	Dermabacter hominis	N/A
Polymicrobial cultures with Gram-negative organisms only
1	N/A	N/A	Pseudomonas aeruginosa	ges, imp, aac(6’)-lb	Pseudomonas aeruginosa	β-lactams, gentamicin
1	N/A	N/A	Pseudomonas aeruginosa	ges, imp, aac(6’)-lb	Pseudomonas putida	Ceftazidime, piperacillin-tazobactam, gentamicin
2	N/A	N/A	Klebsiella spp., Escherichia coli, pan-Enterobacterales	shv, ampC	Escherichia coli	AmpC phenotype
2	N/A	N/A	Klebsiella spp., Escherichia coli, pan-Enterobacterales	shv, ampC	Klebsiella pneumoniae	AmpC phenotype
3	N/A	N/A	Klebsiella spp., Escherichia coli, pan-Enterobacterales	Nil	Escherichia coli	Wild type
3	N/A	N/A	Klebsiella spp., Escherichia coli, pan-Enterobacterales	Nil	Klebsiella oxytoca	Ampicillin resistance
4	N/A	N/A	Klebsiella spp., pan-Enterobacterales	Nil	Klebsiella aerogenes	AmpC phenotype
4	N/A	N/A	Klebsiella spp., pan-Enterobacterales	Nil	Pseudomonas aeruginosa^b	Wild type
Polymicrobial cultures with Gram-positive and Gram-negative organisms
1	Staphylococcus epidermidis, pan Gram-negative	mecA	Escherichia coli, pan-Enterobacterales	TEM	Escherichia coli	Ampicillin
1	Staphylococcus epidermidis, pan Gram-negative	mecA	Escherichia coli, pan-Enterobacterales	TEM	Staphylococcus epidermidis	Not performed
2	Enterococcus faecium, Staphylococcus epidermidis^c	meCA, aac(6’)/aph(2”), ermC, vanA	Pseudomonas aeruginosa	Nil	Pseudomonas aeruginosa	Ceftazidime, piperacillin-tazobactam, meropenem, imipenem
2	Enterococcus faecium, Staphylococcus epidermidis^c	meCA, aac(6’)/aph(2”), ermC, vanA	Pseudomonas aeruginosa	Nil	Enterococcus faecium	Vancomycin, gentamicin
3	Enterococcus faecalis, pan Gram-negative	Nil detected	Klebsiella spp., Escherichia coli, pan-Enterobacterales	shv, ctx-m, tem	Escherichia coli	Ampicillin, cephalosporins
					Klebsiella pneumoniae	Wild type
					Enterococcus faecalis	Wild type
					Enterococcus casseliflavus	Wild type
4	Pan Gram-negative	Nil detected	Escherichia coli pan-Enterobacterales	ctx-m	Escherichia coli	Ampicillin, cephalosporins
					Streptococcus bovis	N/A
5	Enterococcus faecium	aac(6’)/aph(2”)	No pathogen detected		Enterococcus faecium	Wild type
5	Enterococcus faecium	aac(6’)/aph(2”)	No pathogen detected		Elizabethkingia spp.	N/A
6	Enterococcus faecium, pan Gram-negative	aac(6’)/aph(2”)	Not performed as initial Gram-stain was negative for Gram-negative organisms		Enterococcus faecium	Gentamicin
6	Enterococcus faecium, pan Gram-negative	aac(6’)/aph(2”)			Escherichia coli	N/A

^{^a}

N/A, not applicable.

^{^b}

Target organisms which were not identified by BCID kits (false negative).

^{^c}

S. epidermidis identified on BCID testing but not culture. This patient had other cultures that were positive with S. epidermidis. AmpC phenotype: resistant to third-generation cephalosporins and piperacillin-tazobactam but susceptible to cefepime.