Figure 1.

H. capsulatum utilizes a range of amino acids as the sole nitrogen or carbon source.

Growth of wild type Histoplasma yeast in minimal media containing individual amino acids as the sole nitrogen (a) or carbon (b) source. Growth is shown relative to media containing ammonium sulfate (NH₄) for nitrogen (A) or glucose (Glc) for carbon (B). Histoplasma yeast growth was measured via culture optical density at 595 nm after 7 days of growth. Colors correspond to quality of the substrate as a nitrogen (A) or carbon (B) source: red = excellent; orange = good; yellow = fair; green = poor; blue = negligible. Error bars represent standard deviation between 3 biological replicates. Bars annotated with the same letters denote no significant difference between means as determined by one-way ANOVA followed by Tukey’s multiple comparisons test (α = 0.05).