Figure 4.

A Gap3 Dip5 Gai1 triple-transporter deficient strain of Histoplasma grows poorly on most amino acids.

Growth of wild type (“WT,” black circles), gap3 mutant (“gap3,” purple diamonds), DIP5/GAI1-deficient (“dip5 gai1,” green triangles), and triple deficient (“gap3 dip5 gai1,” red squares) Histoplasma yeasts on alanine (a), aspartate (b), glutamine (c), glutamate (d), isoleucine (e), proline (f), casamino acids (g), or glucose and ammonium sulfate (H) as the carbon and nitrogen source(s). Growth was measured as yeast culture optical density at 595 nm over time (hours, x-axis). Error bars represent standard deviation among 3 biological replicates.