Figure B1.

Circularization of the S0756_078 C. difficile genome This figure was generated using Trycyler (Wick et al., 2021) (software v0.5.3) and illustrated by BV‐BRC platform (Olson et al., 2023) (v3.28.9). The different layers from the outside to the inside. Layer 1 represents the CDS forward; Layer 2 represents the CDS reverse; Layer 3 is the RNA genes, the Layer 4 is the antimicrobial resistance (AMR) genes, the Layer 5 is the virulence factors (VF) genes, Layer 6 is the GC content and Layer 7 is the GC skew. The complete genome comprises 4,071,782 bp which contains 3740 genes, including 3541 CDCs. (with protein), 35 rRNAs and 90 tRNAs. This strain contains both virulence operons (PaLoc and CdtLoc). The ribotype 078 is commonly found in animals (Rodriguez et al., 2012) and is the third most common in European hospitals (Abdrabou et al., 2022). The GC content was 29.05%. Ninety‐ nine genes were involved in the stress response, defence, and virulence. This strain has 12 copies of the 16S rRNA gene.