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Fixed cells presented both transferred and endogenous vSAg7. The T-cell hybridomas Omls42.6 (a) and Kmls13.11 (b) were incubated with combinations of untreated or glutaraldehyde-treated CHO/S7 donor cells and CH12.1 APCs, as indicated, and IL-2 production was measured. The cells were fixed, where indicated, with 0.05% glutaraldehyde. (c) T cells were incubated with fixed or nonfixed vSAg7 class II-positive CH12/S7 cells to measure endogenous vSAg7 presentation.
