Figure 7.

Neuronal Ca²⁺ transients are amplified by circSCMH1‐DDX1 interaction in astrocytes after stroke. A) Schematic of AAVs administration and two‐channel miniaturized two‐photon microscope imaging. B) Representative image show dual channel imaging. Scale bar, 20 µm. C) Representative images from stroke mice showing spontaneous GCaMP6s activity in neurons. Scale bar, 100 µm. D) Map of correlation coefficient matrix among 120 neurons. E,F) Quantification of microdomains GCaMP6s signal mean amplitude (E) and mean correlation (F) in dMCAO + EV‐circSCMH1 + AAV‐shRNA‐scramble (253 neurons, 3 mice) and dMCAO+ EV‐circSCMH1 + AAV‐shRNA‐Ddx1 (317 neurons, 3 mice) group. Data are shown as mean ± SEM. ^*** P < 0.001 versus the dMCAO + EV‐circSCMH1 + AAV‐shRNA‐scramble group using the Mann‐Whitney U test.