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. 2024 Oct 23;11(46):2404391. doi: 10.1002/advs.202404391

Figure 8.

Figure 8

EV delivery of circSCMH1 improves motor function recovery after stroke via astrocytic DDX1. A) Schematic of the experimental procedure and behavioural studies. B–D) DDX1 knock down inhibited the improvement of behavioral recovery caused by circSCMH1 intervention by the grid‐walking test (B), cylinder test (C), and adhesive removal test (D). L indicates left forepaw in cylinder test; R, right forepaw in cylinder test; B, both forepaws in cylinder test. n = 12 mice per group. * < 0.05, ** < 0.01, *** < 0.001 versus the dMCAO + AAV‐shRNA‐scramble + EV‐Vector group; # < 0.05, ## < 0.01, ### < 0.001 versus the dMCAO + AAV‐shRNA‐scramble + EV‐circSCMH1 group; two‐way repeated‐measures ANOVA followed by Holm–Sidak post hoc multiple comparison test. E) Strategy for labeling the neurons sparsely and knockdown of Ddx1 specifically in astrocytes to study the dendritic spines through in vivo two‐photon imaging during stroke. F) Representative images of two‐photon spine imaging from mice pre‐stroke and 4 days after the dMCAO model. Scale bar, 10 µm. G) Quantitation of dendritic spine numbers per 10 µm. n = 27 dendrites from 3 mice, *** < 0.001 versus the Pre‐stroke group, ### < 0.001 versus the dMCAO + AAV‐shRNA‐scramble + EV‐Vector group, and $ < 0.05 versus dMCAO + AAV‐shRNA‐scramble + EV‐circSCMH1 group using one‐way ANOVA followed by the Holm‐Sidak test.