FIG. 5.

RNAs synthesized by genomic analogs in which the first transcriptional unit or its mRNA product was designed such that a portion of it could adopt a stem-loop structure. A fluorogram of an agarose-urea gel of actinomycin D-resistant RNAs synthesized in cells transfected with the indicated VSV genomic analog and the VSV N, P, and L support plasmids is shown. RNAs were labeled, extracted, annealed with oligo(dT), and exposed to RNase H as described in Materials and Methods. The N/L mRNA and the I-N/L read-through transcript are shown. The sequences of the inserted transcriptional units and the potential stem-loop structures are shown in Table 1.