TABLE 2.

Examples of methods used (culture media, methods and measurement, expression of results) for the determination of antimicrobial activities toward animal mycoplasmas^a

Mycoplasma species	Culture medium (agar or broth)	Methods and measurement	Expression of resultsb
M. agalactiae	Eaton’s mediumHayflick’s type mediumMycoplasma medium with pyruvatePH mediumPPLO culture medium	Agar dilution: colonies on agarBroth dilution: color changes (sodium pyruvate fermentation), growth in wells after centrifugation (inverted mirror)Etest method: intersection of the inhibition zone with the MIC scaleFlow cytometry: cell counts at different times	MIC per strain, MIC distribution (range), MIC50 and/or MIC90Growth curves (flow cytometry)
M. bovis	Eaton’s mediumFriis mediumHayflick’s type mediumMycoplasma mediumM. bovis mediumPPLO broth	Agar dilution: colonies on agarAgar diffusion: inhibition diametersBroth dilution (prepared or Sensititre plates): color changes (glucose fermentation, sodium pyruvate fermentation, AlamarBlue reagent, redox reagent resazurin), growth in wells after centrifugation (inverted mirror)Etest method: intersection of the inhibition zone with the MIC scaleFlow cytometry: cell counts at different times	Initial and/or final MICMIC per strain, MIC distribution (range), MIC50 and/or MIC90Growth curves (flow cytometry)
M. gallisepticum	FM4 mediumFrey’s mediumFrey’s modified mediumFriis mediumHayflick’s modified medium	Agar dilution: colonies on agarBroth dilution (prepared or Sensititre plates): color changes (glucose fermentation)Etest method: intersection of the inhibition zone with the MIC scale	Initial and/or final MICMIC per strain, MIC distribution (range), MIC50 and/or MIC90Means
M. hyopneumoniae	Difco Turkey Serum (D-TS) mediumFriis mediumFriis modified mediumHank’s-lactalbumin mediumHayflick’s type medium	Agar dilution: colonies on agarAgar diffusion: inhibition diametersBroth dilution (prepared or Sensititre plates): color changes (glucose fermentation)Flow cytometry: cell counts at different timesMicrotiter biphasic agar-broth medium: colonies on agar	Initial and/or final MICMIC per strain, MIC distribution (range), MIC50 and/or MIC90Growth curves (flow cytometry)
M. hyorhinis	Friis mediumFriis modified mediumHayflick’s type mediumM medium	Agar dilution: colonies on agarAgar diffusion: inhibition diametersBroth dilution (prepared or Sensititre plates): color changes (glucose fermentation)	Initial and/or final MICMIC per strain, MIC distribution (range), MIC50 and/or MIC90
M. hyosynoviae	Friis medium with mucinHayflick’s type mediumArginin/mucin-enriched Hayflick’s mediumModified Difco medium with arginine	Agar dilution: colonies on agarBroth dilution (prepared or Sensititre plates): color changes (arginine hydrolysis)	Initial and/or final MICMIC per strain, MIC distribution (range), MIC50 and/or MIC90
M. synoviae	FM4 mediumFrey’s mediumFrey’s modified mediumFriis medium with NAD	Agar dilution: colonies on agarBroth dilution (prepared or Sensititre plates): color changes (glucose fermentation)Etest method: intersection of the inhibition zone with the MIC scale	Initial and/or final MICMIC per strain, MIC distribution (range), MIC50 and/or MIC90

^a These examples were compiled from references 18, 29, 34, 36, 38–43, 47, 49, 56–59, 62–66, 73–76, 81, 86, 87, 93, 94, 98–100, 102, 107–112, 148, 149.

^b MIC₅₀, MIC which inhibits 50% of the tested isolates; MIC₉₀: MIC which inhibits 90% of the isolates tested.