TABLE 6.
Mutations in the 23S rRNA genes and in the ribosomal proteins L4 and L22 conferring resistance in animal Mycoplasma species
| Mutations ina | Mycoplasma species (host species) | Impact on MIC valuesb | References |
|---|---|---|---|
| 23S rRNA domain II | |||
| G748A in one or both alleles (rrl3 and rrl4) | M. synoviae (chicken, turkey) | Increase for Ty (up to 16-fold) and Tm (up to 67-fold) | 82 |
| G748A in both alleles (rrl3 and rrl4) | M. bovis (cattle) | Increase for Ty (up to 64-fold) and Tm (up to 256-fold) | 116, 119, 123 |
| C752T in rrl4 | M. bovis (cattle) | No clear impact | 123 |
| G954A in rrl3 | M. bovis (cattle) | ND: no isolate with only this single mutation | 119 |
| 23S rRNA domain V | |||
| G2057A | M. hyopneumoniae (swine) | Intrinsic resistance to Ery | 92 |
| M. synoviae (chicken, turkey) | Intrinsic resistance to Ery | 82 | |
| M. gallisepticum (chicken) | Increase for Ery (up to 128-fold) | 33, 117 | |
| A2058G in one or both alleles (rrl3 and rrl4) | M. synoviae (chicken, turkey) | Significant increase for Ty (up to 67-fold), Tm (up to 267-fold) and Ln (up to 64-fold) | 82 |
| M. gallisepticum (chicken, turkey) | Significant increase for Ery (up to 8,533-fold), Ty (up to 125-fold), Tm (up to 1,000-fold) and Ln (up to 128-fold) | 33, 78, 117 | |
| M. bovis (cattle) | Significant increase for Ty (up to 32-fold) and Tm (up to 512-fold) | 103, 119, 123 | |
| M. agalactiae (sheep and goats) | Significant increase for Ty (8- to 64-fold) | 111 | |
| A2059G in one or both alleles (rrl3 and rrl4) | M. synoviae (chicken, turkey) | Significant increase for Ty (up to 67-fold), Tm (up to 267-fold), and Ln (up to 64-fold) | 82 |
| M. gallisepticum (chicken, turkey) | Significant increase for Ery, Ty, Tm, and Ln | 33, 78, 117 | |
| M. bovis (cattle) | Significant increase for Ty, Tm, and Ln (up to 32-fold) | 116, 123 | |
| M. agalactiae (sheep and goats) | Significant increase for Ty (320-fold) and Ln (320-fold) | 111 | |
| G2144A in rrl3 | M. bovis (cattle) | No clear impact | 119 |
| C2152 in rrl4 | M. bovis (cattle) | No clear impact | 119 |
| A2503U | M. gallisepticum (in vitro-selected mutants) | ND: mutation always described combined with A2058G or A2059G | 117 |
| G2526A | M. bovis (cattle) | No clear impact | 119 |
| C2611G | M. gallisepticum (chicken) | ND: no isolate with this single mutation | 33 |
| C2611T | M. agalactiae (sheep and goats) | Increase for Ty (3- to 10-fold) and Ln (2-fold) when associated with a A2059G substitution | 111 |
| L4 protein | |||
| G64E | M. synoviae (chicken, turkey) | No clear impact | 82 |
| G185R/W | M. bovis (cattle) | No effect alone | 119 |
| G185A/L/R/V/W | M. bovis (cattle) | No clear impact alone | 123 |
| T186P | M. bovis (cattle) | No clear impact alone | 123 |
| L22 protein | |||
| S89L | M. agalactiae (sheep and goats) | Slight increase for Ty (2- to 8-fold) and no impact for Ln | 111 |
| Q90K/H | M. agalactiae (sheep and goats) | Slight increase for Ty (2-fold) and Ln (2-fold) | 111 |
| M. synoviae (chicken, turkey) | No clear impact | 82 | |
| Q93K/H | M. bovis (cattle) | Increase for Ty (up to 8-fold) and Tm (up to 16-fold) | 119 |
| Q90H | M. bovis (cattle) | No clear impact | 123 |
a
Escherichia coli numbering.
b
A quantitative impact is given into brackets when the MIC increase could be calculated (when the mutation was observed alone and/or when MIC values were available to compare isolates with or without this mutation). Ery, erythromycin; Ty, tylosin; Tm, tilmicosine; Ln, lincomycin; ND, not determined (or impact difficult to evaluate because several mutations were observed at the same time).