FIGURE 4.

Genetic organization of Tn916, Tn5801, ICEBs1, and ICE6013. A comparison of Tn916, Tn5801, ICEBs1, and ICE6013 constructed using EasyFig (233). Amino acid similarity comparisons (20% lower cutoff) using tblastx and are shown as gray shading between regions on each ICE. Genes with likely common functions—regardless of sequence similarity—are similarly colored. Positions of the conjugative origin of transfer (oriT) and single-strand origin of replication (sso) are indicated if known. Recombinase attachment/target sites are indicated by green rectangles. Annotations were collated from previously published figures for each ICE (15, 181, 197, 204, 206, 234, 235).